On the basis of inverse PCR, we have founded a IPCR technique to clone foreign gene's flanking sequence in transgenic rice, which is suitable to treat mass materials.

以反向pcr (IPCR)为基础建立了适合于处理大量材料的克隆转基因水稻中外源基因旁侧序列的技术体系。

Objective: To investigate the information of transgene integrations in mice mediated with lentiviral vectors, the inverse PCR was used to clone the sequences of integration sites.

目的：为分析慢病毒介导的转基因小鼠中外源基因整合位点的信息，应用反向PCR克隆整合位点序列。