No degradation of otilonium was found in acetonitrile.

在乙腈中未发现降解。

The mobile phase consisted acetonitrile - water( 1:4).

以乙腈-水（1：4）为流动相；

Mobile phase was acetonitrile - phosphate buffer (27: 73);

流动相为乙睛-磷酸盐缓冲液（27：73）；

Acetonitrile and polymer are metabolic toxin at all the time.

乙腈和聚合物始终为代谢毒素。

The SPE column was washed with water and eluted with acetonitrile.

用水淋洗、乙腈洗脱固相萃取柱。