The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.

表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。

Methods A combination of euglobulin precipitation, ion-exchange chromatography, (NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法采用优球蛋白沉淀、离子交换色谱、（NH4）2SO4沉淀和亲和色谱法进行纯化。

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human CDK5. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由合成的人类CDK5氨基酸序列对应肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Antibodies are purified by protein A and peptide affinity chromatography.

抗体由蛋白A和肽段亲和层析纯化得到。

Cu(II)-immobilized metal affinity chromatography (Cu(II)-IMAC), as a highly reliable analytical procedure, has been widely used in separation and purification of biomacromolecule.

铜离子固定金属亲和色谱作为一种有效的分析方法，已普遍应用于生物大分子的分离与纯化。

The application of molecular recognition in affinity chromatography, solid extraction, membrane separation technology raises the separation efficiency and separates those optical isomeric compounds.

分子识别应用于亲和色谱、固相萃取、膜分离技术极大地促进了分离效率的提高和一些复杂物质的拆分。

Transformants were induced by IPTG, and then expressed. By DTT reduction, the soluble 19 peptide was obtained from chitin affinity chromatography.

表达的融合蛋白经几丁质亲和层析、二硫苏糖醇（DTT）的柱内还原，直接获得可溶性19肽。

Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质空间分离的蛋白经过镍住得到了进一步的纯化。

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PRAS40. Antibodies are purified by peptide affinity chromatography.

该多克隆抗体是由合成的人源的针对PRAS40 蛋白氨基酸序列的肽段免疫动物而生产的。抗体由肽亲和层析技术纯化。

The defensin protein was purified by affinity chromatography using the self-cleavable fusion protein.

然后，通过亲和层析利用自剪切融合蛋白分离提纯目的蛋白。

In the present study, recombinant human IRF-7 was expressed in Escherichia coli, purified by affinity chromatography, and utilized to raise polyclonal antibody in BALB/c mice.

本文在大肠杆菌中表达了重组IRF-7，利用亲和层析的方法进行了纯化，并制备了鼠抗IRF-7的多克隆抗体。

The trypsin was covalently linked with chemical modified granule chitosan and was used to isolate and purify aprotininum from the extract of cattle lungs by affinity chromatography.

采用琼脂糖凝胶为载体，共价偶联牛胰蛋白酶，制成抑肽酶亲和吸附剂，将其用于亲和层析牛肺提取液，分离纯化高比活抑肽酶。

The purity of inclusion bodies was above 70% and that of protein purified by nickel affinity chromatography was 95%.

提纯的包涵体纯度可达70%以上，用镍亲和层析方法纯化蛋白则可达到95%。

Expressed proteins as inclusion bodies were solubilized, refolded and purified by GST affinity chromatography.

表达产物为包涵体形式，溶解和折叠后，以GST亲合层析色谱纯化。

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human CENP-A protein. Antibodies are purified by peptide affinity chromatography.

通过合成的与人源CENP-A蛋白相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过多肽亲和层析纯化获得。

PurposeThe aim is to purify human platelet factor 4 (PF4) using a monoclonal antibody affinity chromatography.

目的用单克隆抗体亲和色谱从人血小板破碎液中纯化血小板第4因子（PF4 ）。

Affinity chromatography is a powerful tool used in the purification of antibodies. In this paper, different kinds of affinity ligands used in affinity chromatography are described.

亲和色谱是用于抗体纯化的有效方法，本文对亲和色谱配体的研究进展进行了综述。

Incorporating with appropriate transport model equation, the SMA model was used to model the dye-ligand affinity chromatography process.

结合适当的传质模型方程，将SMA模型应用于色素亲和色谱过程模拟。

Methods Human sperm mannose receptors were purified by mannose-agarose gel affinity chromatography coloumn.

目的分离纯化人精子甘露糖受体并测定其分子量和等电点。

Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.

建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。

Along with the development in the very field, novel types of affinity chromatography emerged in recent years, and its range of application expanded significantly as well.

随着技术的发展，出现了多种新型的亲和色谱，其应用范围也不断拓展。

Both proteins were purified with Nickel chelate affinity chromatography.

经金属螯合层析纯化，获得了高纯度的表达蛋白。

Highly purified preparation of this protein was obtained through purification with affinity chromatography.

亲和层析获得了高纯度的蛋白。

Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be easily purified by Metal Chelated Affinity Chromatography(MCAC).

研究了应用金属螯合亲和层析法纯化重组类人胶原蛋白的条件。

The soluble expression products was purified by 50%(φ) Ni-NTA affinity chromatography and used as an antigen, AIV antibody could be detected by ELISA method.

将可溶表达的融合蛋白用体积分数为50%的N i-NTA树脂过柱纯化，用融合蛋白作抗原，通过ELISA方法，能特异性地检测出禽流感阳性血清。

Among all purification methods, affinity chromatography shows the excellent characteristics such as high selectivity, high yield, etc.

以亲和技术为基础的分离纯化方法与其它分离制备方法相比具有高选择性、高活力回收等优点。