The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene.

用双引物法对GI基因进行体外定点突变，构建了GI突变体g 138p。

Human brain derived neurotrophic factor gene was used as template for oligonucleotide based site directed mutagenesis and PCR.

应用寡核苷酸诱导的定点突变和PCR技术对人脑源性神经营养因子基因进行突变，并完成了测序鉴定。

AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.

目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。

Site-directed mutagenesis is one of the key methods used to study gene function.

定点突变是研究基因功能的一种关键方法。

Objective: To develop a simple and efficient way to perform site-directed mutagenesis.

目的：介绍一种简便、有效的定点突变技术。