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Methods Routine physiological and biochemical methods and real time PCR were used.
方法常规生理生化鉴定法和实时荧光pcr法。
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Objective to examine the value of real time PCR in the diagnosis of cytomegalovirus pneumonia in renal Allograft Recipient.
目的探讨实时荧光定量pcr检测巨细胞病毒在肾移植术后肺部感染诊断中的应用价值。
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Objective The serum HBV-DNA has been extensively detected by fluorescence-quantitative real time PCR (FQ-PCR) in our country.
目的目前国内较多的医院采用荧光定量的方法对血清中的HBV - DNA进行定量检测。
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To develop a fluorescent MGB probe real time PCR platform for detection the mutation of phenylalanine hydroxylase gene in patients with phenylketonuria.
探讨荧光mgb探针实时P CR技术检测经典型苯丙酮尿症的基因突变。
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The facility provides technical services for the clients, which include Oligonucleotide Synthesis, Protein Sequencing, DNA Sequencing, Real Time PCR/Allele Detection.
该 机构为客户提供寡核苷酸合成、 蛋白质测序、 DNA测序、实时PCR和等位 基因检测等技术服务。
实时荧光定量PCR (Quantitative Real-time PCR)是一种在DNA扩增反应中,以荧光化学物质测每次聚合酶链式反应(PCR)循环后产物总量的方法。通过内参或者外参法对待测样品中的特定DNA序列进行定量分析的方法。· Real-timePCR是在PCR扩增过程中,通过荧光信号,对PCR进程进行实时检测。由于在PCR扩增的指数时期,模板的Ct值和该模板的起始拷贝数存在线性关系,所以成为定量的依据。