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The transcripts were analyzed by Northern blotting with various probes.
通过不同探针的 RNA 印迹技术分析转录本。
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Results: After transfection of the antisense ERCC1, Northern and Western blotting indicated that the RNA and protein expression level of ERCC1 was obviously decreased.
结果: 卵巢癌细胞转染反义ERCC1基因后,ERCC1基因转录水平、蛋白表达水平明显下降。
Northern杂交(Northernblotting)1979年,J.C.Alwine等提出:将电泳凝胶中的RNA转移到叠氮化的或其他化学修饰的活性滤纸上,通过共价交联作用使它们结合,因其方法同Southern杂交十分相似,故称之为Northern杂交。