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METHODS: Gel filtration chromatography was adopted.
方法:采用凝胶色谱法。
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Objective to find out the optimal condition for separation of the protein mixture by gel filtration chromatography.
目的探讨凝胶过滤层析分离蛋白质的最佳实验效果。
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Selenium-containing phycocyanin(Se-PC)was purified from Se-enriched Spirulina platensis using ion exchange and gel filtration chromatography.
使用离子交换和凝胶过滤色谱法从富硒螺旋藻中提纯含硒藻蓝蛋白。
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The changes of the molecular weight distributions of xylan during enzymatic hydrolysis have been investigated with gel filtration chromatography.
采用凝胶过滤色谱法研究了木聚糖在酶解过程中分子量分布的变化。
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Methods: The gel filtration chromatography with Sephadex G-25 was used to separate different components from the small intestine extracts of sheep.
方法:葡聚糖凝胶分离及反相高效液相色谱法提纯 羊小肠活性抗菌肽;
凝胶过滤层析(gel filtration chromatography)法又称排阻层析或分子筛方法,主要是根据蛋白质的大小和形状,即蛋白质的质量进行分离和纯化。层析柱中的填料是某些惰性的多孔网状结构物质,多是交联的聚糖(如葡聚糖或琼脂糖)类物质,使蛋白质混合物中的物质按分子大小的不同进行分离。也叫做分子排阻层析(molecular-exclusion chromatography)。一种利用带孔凝胶珠作基质,按照分子大小分离蛋白质或其它分子混合物的层析技术。 一般是大分子先流出来,小分子后流出来。